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du145 human prostate cancer cell strains  (ATCC)


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    ATCC du145 human prostate cancer cell strains
    Concentration-response curves of compounds V1–V6 on prostate cancer cell lines (PC3 and <t>DU145).</t> Cells were treated with increasing concentrations of each compound for 48 h. Data are expressed as mean ± SEM of N = 3 independent biological replicates, each is the average of three technical replicates.
    Du145 Human Prostate Cancer Cell Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8410 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/du145 human prostate cancer cell strains/product/ATCC
    Average 99 stars, based on 8410 article reviews
    du145 human prostate cancer cell strains - by Bioz Stars, 2026-05
    99/100 stars

    Images

    1) Product Images from "Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer"

    Article Title: Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer

    Journal: RSC Advances

    doi: 10.1039/d5ra09216g

    Concentration-response curves of compounds V1–V6 on prostate cancer cell lines (PC3 and DU145). Cells were treated with increasing concentrations of each compound for 48 h. Data are expressed as mean ± SEM of N = 3 independent biological replicates, each is the average of three technical replicates.
    Figure Legend Snippet: Concentration-response curves of compounds V1–V6 on prostate cancer cell lines (PC3 and DU145). Cells were treated with increasing concentrations of each compound for 48 h. Data are expressed as mean ± SEM of N = 3 independent biological replicates, each is the average of three technical replicates.

    Techniques Used: Concentration Assay

    Effects of flavonoids on mitochondrial membrane potential (MMP) in PC3 and DU145 prostate cancer cell lines. MMP was assessed using rhodamine 123 fluorescence intensity, expressed as relative fluorescence units (RFU), and normalized to the untreated control group (100%). Lower percentages indicate greater MMP depolarization. Cells were treated with two concentrations (50 µM and 100 µM) of each compound. Data are presented as mean ± SEM of N = 3 independent biological replicates, each is the average of three technical replicates. Statistical significance ( p < 0.05) of treatment effect compared to control (untreated cells) was determined using two-way ANOVA with Sidak's multiple comparisons test. *** p < 0.001.
    Figure Legend Snippet: Effects of flavonoids on mitochondrial membrane potential (MMP) in PC3 and DU145 prostate cancer cell lines. MMP was assessed using rhodamine 123 fluorescence intensity, expressed as relative fluorescence units (RFU), and normalized to the untreated control group (100%). Lower percentages indicate greater MMP depolarization. Cells were treated with two concentrations (50 µM and 100 µM) of each compound. Data are presented as mean ± SEM of N = 3 independent biological replicates, each is the average of three technical replicates. Statistical significance ( p < 0.05) of treatment effect compared to control (untreated cells) was determined using two-way ANOVA with Sidak's multiple comparisons test. *** p < 0.001.

    Techniques Used: Membrane, Fluorescence, Control

    The representative dot plots display how the viable cells (Annexin V − /PI − ), early apoptotic cells (Annexin V + /PI − ), late apoptotic cells (Annexin V + /PI + ), and necrotic cells (Annexin V − /PI + ) were distributed after the 24-hours treatment. The control group was treated with the vehicle only. Effect of selected compounds (V3, V5, and V6) on apoptosis induction in PC3 and DU145 prostate cancer cells as assessed by Annexin V/Propidium Iodide.
    Figure Legend Snippet: The representative dot plots display how the viable cells (Annexin V − /PI − ), early apoptotic cells (Annexin V + /PI − ), late apoptotic cells (Annexin V + /PI + ), and necrotic cells (Annexin V − /PI + ) were distributed after the 24-hours treatment. The control group was treated with the vehicle only. Effect of selected compounds (V3, V5, and V6) on apoptosis induction in PC3 and DU145 prostate cancer cells as assessed by Annexin V/Propidium Iodide.

    Techniques Used: Control

    Annexin V/PI analysis of apoptosis in PC3 and DU145 prostate cancer cells treated with flavone derivatives. DU145 cells were treated with 10 µM V3 (4′-hydroxy-3,5,6,7-tetramethoxyflavone), V5, or V6 for 24 h and stained with Annexin V-FITC/propidium iodide. Flow cytometric analysis quantified the percentage of viable, early apoptotic, and late apoptotic cells. Data were analyzed by two-way ANOVA with Sidak multiple comparisons test. Data are expressed as mean ± SEM of three independent experiments performed in triplicate. * p < 0.05, ** p < 0.01; ns, not significant.
    Figure Legend Snippet: Annexin V/PI analysis of apoptosis in PC3 and DU145 prostate cancer cells treated with flavone derivatives. DU145 cells were treated with 10 µM V3 (4′-hydroxy-3,5,6,7-tetramethoxyflavone), V5, or V6 for 24 h and stained with Annexin V-FITC/propidium iodide. Flow cytometric analysis quantified the percentage of viable, early apoptotic, and late apoptotic cells. Data were analyzed by two-way ANOVA with Sidak multiple comparisons test. Data are expressed as mean ± SEM of three independent experiments performed in triplicate. * p < 0.05, ** p < 0.01; ns, not significant.

    Techniques Used: Staining

    Cell cycle profile following treatment with flavonoids V3. (A) A representative Kaluza cell cycle histogram of PI-stained PC3 and DU145 cells following 24 h-treatment of flavonoid V3 (10 µM) compared to untreated controls. (B–D) Progression of cell cycle phases assessed using ordinary one-way ANOVA with Dunnett's multiple comparisons test. Bars represent mean ± SEM of 2 independent biological replicates.
    Figure Legend Snippet: Cell cycle profile following treatment with flavonoids V3. (A) A representative Kaluza cell cycle histogram of PI-stained PC3 and DU145 cells following 24 h-treatment of flavonoid V3 (10 µM) compared to untreated controls. (B–D) Progression of cell cycle phases assessed using ordinary one-way ANOVA with Dunnett's multiple comparisons test. Bars represent mean ± SEM of 2 independent biological replicates.

    Techniques Used: Staining



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    du145 human prostate cancer cell strains  (ATCC)
    99
    ATCC du145 human prostate cancer cell strains
    Concentration-response curves of compounds V1–V6 on prostate cancer cell lines (PC3 and <t>DU145).</t> Cells were treated with increasing concentrations of each compound for 48 h. Data are expressed as mean ± SEM of N = 3 independent biological replicates, each is the average of three technical replicates.
    Du145 Human Prostate Cancer Cell Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/du145 human prostate cancer cell strains/product/ATCC
    Average 99 stars, based on 1 article reviews
    du145 human prostate cancer cell strains - by Bioz Stars, 2026-05
    99/100 stars
    		  Buy from Supplier

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    Concentration-response curves of compounds V1–V6 on prostate cancer cell lines (PC3 and DU145). Cells were treated with increasing concentrations of each compound for 48 h. Data are expressed as mean ± SEM of N = 3 independent biological replicates, each is the average of three technical replicates.

    Journal: RSC Advances

    Article Title: Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer

    doi: 10.1039/d5ra09216g

    Figure Lengend Snippet: Concentration-response curves of compounds V1–V6 on prostate cancer cell lines (PC3 and DU145). Cells were treated with increasing concentrations of each compound for 48 h. Data are expressed as mean ± SEM of N = 3 independent biological replicates, each is the average of three technical replicates.

    Article Snippet: PC3 and DU145 human prostate cancer cell strains are sourced from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

    Techniques: Concentration Assay

    Effects of flavonoids on mitochondrial membrane potential (MMP) in PC3 and DU145 prostate cancer cell lines. MMP was assessed using rhodamine 123 fluorescence intensity, expressed as relative fluorescence units (RFU), and normalized to the untreated control group (100%). Lower percentages indicate greater MMP depolarization. Cells were treated with two concentrations (50 µM and 100 µM) of each compound. Data are presented as mean ± SEM of N = 3 independent biological replicates, each is the average of three technical replicates. Statistical significance ( p < 0.05) of treatment effect compared to control (untreated cells) was determined using two-way ANOVA with Sidak's multiple comparisons test. *** p < 0.001.

    Journal: RSC Advances

    Article Title: Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer

    doi: 10.1039/d5ra09216g

    Figure Lengend Snippet: Effects of flavonoids on mitochondrial membrane potential (MMP) in PC3 and DU145 prostate cancer cell lines. MMP was assessed using rhodamine 123 fluorescence intensity, expressed as relative fluorescence units (RFU), and normalized to the untreated control group (100%). Lower percentages indicate greater MMP depolarization. Cells were treated with two concentrations (50 µM and 100 µM) of each compound. Data are presented as mean ± SEM of N = 3 independent biological replicates, each is the average of three technical replicates. Statistical significance ( p < 0.05) of treatment effect compared to control (untreated cells) was determined using two-way ANOVA with Sidak's multiple comparisons test. *** p < 0.001.

    Article Snippet: PC3 and DU145 human prostate cancer cell strains are sourced from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

    Techniques: Membrane, Fluorescence, Control

    The representative dot plots display how the viable cells (Annexin V − /PI − ), early apoptotic cells (Annexin V + /PI − ), late apoptotic cells (Annexin V + /PI + ), and necrotic cells (Annexin V − /PI + ) were distributed after the 24-hours treatment. The control group was treated with the vehicle only. Effect of selected compounds (V3, V5, and V6) on apoptosis induction in PC3 and DU145 prostate cancer cells as assessed by Annexin V/Propidium Iodide.

    Journal: RSC Advances

    Article Title: Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer

    doi: 10.1039/d5ra09216g

    Figure Lengend Snippet: The representative dot plots display how the viable cells (Annexin V − /PI − ), early apoptotic cells (Annexin V + /PI − ), late apoptotic cells (Annexin V + /PI + ), and necrotic cells (Annexin V − /PI + ) were distributed after the 24-hours treatment. The control group was treated with the vehicle only. Effect of selected compounds (V3, V5, and V6) on apoptosis induction in PC3 and DU145 prostate cancer cells as assessed by Annexin V/Propidium Iodide.

    Article Snippet: PC3 and DU145 human prostate cancer cell strains are sourced from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

    Techniques: Control

    Annexin V/PI analysis of apoptosis in PC3 and DU145 prostate cancer cells treated with flavone derivatives. DU145 cells were treated with 10 µM V3 (4′-hydroxy-3,5,6,7-tetramethoxyflavone), V5, or V6 for 24 h and stained with Annexin V-FITC/propidium iodide. Flow cytometric analysis quantified the percentage of viable, early apoptotic, and late apoptotic cells. Data were analyzed by two-way ANOVA with Sidak multiple comparisons test. Data are expressed as mean ± SEM of three independent experiments performed in triplicate. * p < 0.05, ** p < 0.01; ns, not significant.

    Journal: RSC Advances

    Article Title: Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer

    doi: 10.1039/d5ra09216g

    Figure Lengend Snippet: Annexin V/PI analysis of apoptosis in PC3 and DU145 prostate cancer cells treated with flavone derivatives. DU145 cells were treated with 10 µM V3 (4′-hydroxy-3,5,6,7-tetramethoxyflavone), V5, or V6 for 24 h and stained with Annexin V-FITC/propidium iodide. Flow cytometric analysis quantified the percentage of viable, early apoptotic, and late apoptotic cells. Data were analyzed by two-way ANOVA with Sidak multiple comparisons test. Data are expressed as mean ± SEM of three independent experiments performed in triplicate. * p < 0.05, ** p < 0.01; ns, not significant.

    Article Snippet: PC3 and DU145 human prostate cancer cell strains are sourced from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

    Techniques: Staining

    Cell cycle profile following treatment with flavonoids V3. (A) A representative Kaluza cell cycle histogram of PI-stained PC3 and DU145 cells following 24 h-treatment of flavonoid V3 (10 µM) compared to untreated controls. (B–D) Progression of cell cycle phases assessed using ordinary one-way ANOVA with Dunnett's multiple comparisons test. Bars represent mean ± SEM of 2 independent biological replicates.

    Journal: RSC Advances

    Article Title: Context-dependent cytotoxicity and ADMET profiling of methoxylated flavonoids as novel leads for metastatic prostate cancer

    doi: 10.1039/d5ra09216g

    Figure Lengend Snippet: Cell cycle profile following treatment with flavonoids V3. (A) A representative Kaluza cell cycle histogram of PI-stained PC3 and DU145 cells following 24 h-treatment of flavonoid V3 (10 µM) compared to untreated controls. (B–D) Progression of cell cycle phases assessed using ordinary one-way ANOVA with Dunnett's multiple comparisons test. Bars represent mean ± SEM of 2 independent biological replicates.

    Article Snippet: PC3 and DU145 human prostate cancer cell strains are sourced from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

    Techniques: Staining